基因重组蛋白Recombinant protein FACTORS

基因重组蛋白Recombinant protein FACTORS

技术服务详细描述

We offers full Technology-Transfer packages, which include a comprehensive course at our facility. The company has developed a simple, cost effective and easy to implement proprietary Technology for the production of highly active bulk recombinant E.Coli derived proteins.

Each batch production at our facility is within 2-3 days and can yield 1000-2000 mg/protein.

The Technologies offered give a clear edge over the competition resulting from low production costs, high yield and production efficiency.

We are also seeking for strategic partners and alliances in the biotech and pharmaceutical field to develop and market bio-drugs according to FDA and EU pharma regulations and certification.

We offer the following recombinant technologies:

Product Description

 rHuIFN-a 2a

 rHuIFN-alpha 2b

 rHuIFN-gamma

 rHuG-CSF

 rHuGM-CSF

 rHuIL-2

 rHuGH

 rHuIGF-I

 rHuEGF

 rHuFGF-basic

 rHuIL-11

Interferon-Alpha 2a

Interferon-Alpha 2b

Interferon gamma 1b

Granulocyte Colony Stimulating Factor

Granulocyte Macrophage Colony Stimulating Factor

Interleukin-2

Growth Hormone

Insulin Like Growth Factor

Epidermal Growth Factor

Fibroblast Growth Factor

Interleukin-11

For more technology offers please contact us at info@prospec.co.il

Brief description of technology transfer package:

1. Introduction.

2. Manufacturing.

    2.1 General.

    2.2 Construction of the plasmid expressing of the protein.

    2.3 DNA sequence.

    2.4 Expression of the protein in E.coli.  

3. Biosynthesis of the protein substance.

    3.1 Fermentation process:

    3.2 Inoculum.

    3.3 Seed fermentor.

    3.4 Production fermentor.

    3.5 Fermentor Harvest.

    3.6 Cell disruption.

3.7Water Washes.

4. Purification of the protein from inclusion bodies

    4.1 Dissolution of the protein inclusion bodies.

    4.2 Dilution and refolding of the protein.

    4.3 Clarification and concentration.

    4.4 Enzymatic removal of the N-terminal methionine (if needed).

    4.5 Purification of the protein by preparative RP-HPLC (if needed).

    4.6 Purification of the protein by ion-exchange Chromatography (if needed).

    4.7 Dialysis and Lyophilization.     

5. Detailed characterization of the protein

    5.1 Polyacrylamide gel electrophoresis.

    5.2 UV spectroscopy.

    5.3 N-terminal amino acid sequence determination.

    5.4 Analytical reversed-phase HPLC.

    5.5 Biological activity in-vitro.

6. Characterization of impurities

     6.1 Endotoxin content by LAL.

    If you have any requirement, please don't hesitate to contact us!

上海卓康生物科技有限公司

联系电话：021－55660344，021－55063976

传真：021－55660344

网址：www.elisabio.com     网址：www.antibodybio.com

联系人：黄力，业务qq: 909447355